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KMID : 0043320100330121953
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Archives of Pharmacal Research
2010 Volume.33 No. 12 p.1953 ~ p.1958
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Spirafolide from bay leaf (Laurus nobilis) prevents dopamine-induced apoptosis by decreasing reactive oxygen species production in human neuroblastoma SH-SY5Y cells
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Ham Ah-rom
Kim Bo-Ra
Koo Uk
Nam Kung-Woo
Lee Sung-Jin
Kim Kyeong-Ho
Shin Jong-Heon
Mar Woong-Chon
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Abstract
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Reactive oxygen species (ROS) are important mediators in many neurodegenerative diseases including Alzheimer’s disease and Parkinson’s disease. This study tested the neuroprotective effects of spirafolide, a compound purified from the leaves of Laurus nobilis L. (Lauraceae), against dopamine (DA)-induced apoptosis in human neuroblastoma SH-SY5Y cells. Following a 24-h exposure of cells to DA (final conc., 0.6 mM), we observed a marked increase in apoptosis, increased generation of ROS and decreased cell viability. Pretreatment of the cells for 24 h with spirafolide (0.4, 2, and 10 μM) before exposure to DA notably increased cell survival (p < 0.01) and lowered intracellular ROS levels (p < 0.01). These results indicate that spirafolide has neuroprotective effects against DA toxicity. These effects may contribute to the treatment of neurodegenerative diseases.
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KEYWORD
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Laurus nobilis, Spirafolide, Reactive oxygen species, Apoptosis, SH-SY5Y cells
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